directory HOME > products > cell culture > Live Cell Imaging Fluidic System
Live Cell Imaging Fluidic System - Takasago Fluidic Systems
Live Cell Imaging Fluidic System
Fully-automated Medium and Reagent Pipetting
System for Live Cell Imaging
Features
1
Easily programmable from rapid medium exchange to gentle perfusion. Researchers can simply press “Start” to begin fully-automatic pipetting and focus entirely on the observation of cells.2
Capable of slow and precise reagent addition (uL/min level), which is difficult with conventional manual pipetting. A controlled supply speed results in stable images.*A certain level of pulsation appears when peristaltic pumps are used.
3
Gradient mode can control concentration by gradually changing the flow rates of the 2 perfusion pumps. (Only available for CEIM-0200 Series)4
Maximum of 4 types of reagent addition pump are available with different flow rates. Please contact us for more information.Application Example
- Feed the HeLa cells into the glass-bottom dish, and transfect the genes.
- After incubating for one day, add 1ml of reagent to stimulate the genes while photographing.
- The change in fluorescence wavelength of GEM-GECO1 inside the cells (when GEM-GECO1 is chemically bonded to calcium ion, the color of the fluorescence wavelength shifts from green to blue) was observed.
[Experiment Conditions]
Microscope: Inverted confocal laser microscope
Magnification of objective lens: 60x
Numerical aperture of objective lens: 1.35
Cells: HeLa cells
Reagent: ionomycin
Data courtesy of Dr. H. Shibata and Mr. T. Hayashimoto, Molecular and Cellular Regulation Lab., Graduate School of Bioagricultural Sciences, Nagoya University (Japan)
System Configuration
PC Setting （Example of Medium Exchange Mode）
Processes under this setting:
Process 1: Add 0.1ml of reagent A to the dish. Pause for 10 seconds.
Process 2: Waste 2.0ml of medium/reagent mixture.
Process 3: Feed 2.0ml of new medium to the dish. Pause for 10 seconds.
Process 4: Waste 2.0ml of medium.
Process 5: Add 2.0ml of new medium to the dish.
Specifications
| Model Number | CEIM-010x | CEIM-020x |
|---|---|---|
| Medium Feed／Waste Pump Flow Rate | 3 ~ 10ml/min | |
| Reagent Addition Pump Flow Rate | 0.45 ~ 7ml/min | 0.1ul ~ 7ml/min |
| Gradient Mode | × | ○ |
| Dimensions | 100 × 240 × 100 mm | |
| Supported OS | Windows7 and later | |
| Appropriate Dish Diameter | 35mm | |
Note: Details including specifications may change without notification.
