TFS Takasago Fluidic Systems

  • Live Cell Imaging Fluidic System
  • Live Cell Imaging Fluidic System
  • Live Cell Imaging Fluidic System
  • Live Cell Imaging Fluidic System
  • Live Cell Imaging Fluidic System
  • Live Cell Imaging Fluidic System

Live Cell Imaging Fluidic System

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FEATURES / CHARACTERISTICS

Fully-automated Medium and Reagent Pipetting System for Live Cell Imaging.

It meets the standard ANSI/SBS footprint and fits in the Stage Top® Incubator. You can easily set up various protocols on your PC, and the system will automatically conduct the pipetting processes you currently carry out manually. The pump heads and other wetted parts are disposable.

Features

  1. Easily programmable from rapid medium exchange to gentle perfusion. Researchers can simply press “Start” to begin fully-automatic pipetting and focus entirely on the observation of cells.
  2. Capable of slow and precise reagent addition (uL/min level), which is difficult with conventional manual pipetting. A controlled supply speed results in stable images.
    *A certain level of pulsation appears when peristaltic pumps are used.
  3. Gradient mode can control concentration by gradually changing the flow rates of the 2 perfusion pumps. (Only available for CEIM-0200 Series)
  4. Maximum of 4 types of reagent addition pump are available with different flow rates. Please contact us for more information.

Application Example

As the dosing action doesn’t disturb imaging, even though using with high powered / high NA microscope (60X, NA1.35), it is possible to clearly observe the images of before/after dosing the reagent.

live cell imaging Application Example

  1. Feed the HeLa cells into the glass-bottom dish, and transfect the genes.
  2. After incubating for one day, add 1ml of reagent to stimulate the genes while photographing.
  3. The change in fluorescence wavelength of GEM-GECO1 inside the cells (when GEM-GECO1 is chemically bonded to calcium ion, the color of the fluorescence wavelength shifts from green to blue) was observed.

[Experiment Conditions]


Microscope: Inverted confocal laser microscope
Magnification of objective lens: 60x
Numerical aperture of objective lens: 1.35
Cells: HeLa cells
Reagent: ionomycin

Data courtesy of Dr. H. Shibata and Mr. T. Hayashimoto, Molecular and Cellular Regulation Lab., Graduate School of Bioagricultural Sciences, Nagoya University (Japan)

System Configration

live cell imaging System Configuration

 PC Setting (Example of Medium Exchange Mode)

live cell imaging PC setting

Processes under this setting:


Process 1: Add 0.1ml of reagent A to the dish. Pause for 10 seconds.
Process 2: Waste 2.0ml of medium/reagent mixture.
Process 3: Feed 2.0ml of new medium to the dish. Pause for 10 seconds.
Process 4: Waste 2.0ml of medium.
Process 5: Add 2.0ml of new medium to the dish.

Video of Live Cell Imaging Fluidic System

 

Features
Model Number CEIM-010x CEIM-020x
Medium Feed/Waste Pump Flow Rate 3 ~ 10ml/min
Reagent Addition Pump Flow Rate 0.45 ~ 7ml/min 0.1ul ~ 7ml/min
Gradient Mode ×
Dimensions 100 × 240 × 100 mm
Supported OS Windows7 and later
Appropriate Dish Diameter 35mm

Note: Details including specifications may change without notification.
Stage Top® is a registered trademark of Tokai Hit Co., LTD.
Windows® is a registered trademark of Microsoft Corporation in the US and other countries.

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