Suitable for applications where constant and frequent medium replacement is required. (e.g. iPS cell culture)
Automatic medium replacement
The culture medium is replaced automatically at a specified interval (12, 24, 48, or 72 hours*). *Can be customized.
Can be used in an incubator
The system can operate continuously for up to 7 days with batteries, and can be placed in an incubator without any wiring.
Can be placed on a stage of microscope
The 6-well plate can remain in the system and be placed on a microscope stage when you observe the cells, which also reduces environmental changes.
All system components that are exposed to the medium are replaceable.
Note: Details such as specifications, etc. may be changed without notice.
Case 1. hiPSC (Human induced Pluripotent Stem Cells)
Colony growth was successfully observed for 4 days after passage (engraftment), during which the culture medium was automatically replaced by our system.
- Cells: 253G1 Human iPS cells (NHDF reprogrammed by 3 factors; Oct3/4, Sox2, Klf4)
- Feeder Cells: MMC treated SNL76/7 cells
- Medium: DMEM-12 (including KSR, NEAA, PSG, 2-ME, bFGF)
[Data courtesy of Kidoaki S. Lab, Institute for Materials Chemistry and Engineering, Kyushu University (Japan)]
Case 2. Embryoid Bodies (EB) of mouse iPS cells
iPS cells were cultured in Knockout D-MEM based myocardial differentiation medium using our system, and pulsation of differentiation-inducted cardiomyocytes derived from mouse iPS cells was observed.
[Data courtesy of Fujii T. Lab, Institute of Industrial Science, The University of Tokyo (Japan)]
Case 3. BrainXell
BrainXell’s summary on comparative experiment between Takasago’s Portable Medium Replacement System and traditional medium replacement methods in induced pluripotent stem cells (iPSCs) maintenance. Download more detail
[Data courtesy of Ms. Sondra Minter, BrainXell, MADISON, WI]
VIDEO OF PORTABLE MEDIUM REPLACEMENT SYSTEM
Q. How much medium can be replaced?
A. The volume of medium is 3.0 mL/well during culture, of which Approx. 2.7 mL/well must be replaced (Only if using Takasago recommended well plate).
Note: If Takasago recommended well plates are not used, the well depth could be different, resulting in different replacement medium volume. The medium replacement volume can be customized as an option.
Q. What are the wetted parts made of?
A. SUS304 (nozzle), PP (bottle), PP (straw), silicone (tube).
Q. How frequently is the medium replaced?
A. Every 12, 24, 48 and 72 hours are our basic setting. The interval setting can be customized.
Q. What should be used as the power supply?
A. AC adapter or two AA alkaline batteries.
Note: Do not use nickel-metal hydride (NiMH) batteries because the controller may be damaged by the gas generated.
Q. What is the capacity of the reservoir bottle?
A. 125 mL for the standartd set, which corresponds to the volume of 5 times replacement.
You can choose 15ml conical tubes as an option.
Q. When should I replace the batteries?
A. We have confirmed 7 consecutive days operation, under 24-hour medium replacement interval. However, we recommend early replacement.
Q. Which type of well plate is adaptable?
A. Thermo Fisher Scientific Inc.: NUNC # 140675 and Sumitomo Bakelite
MS-80060 are our recommendation.
Note: A common 6-well plate can also be used, but the residual medium volume may change.
Q Is there any application example?
A Cardio myogenic differentiation of iPS cells, culture of chicken cardiomyocytes, and colony formation in undifferentiated iPS cells.
Q. Is it possible to use in a CO2 incubator?
Q. What is the reliability of replacement fluid volume?
A. Less than 10% /well.
Q. How much is the flow rate of the pump?
A. Approximately 0.8 mL/min per tubing.
Q. Is it possible to autoclave the disposable components and bottles?
A. Basically we do not recommend. Although, autoclavable materials are basically used the well plate top cover (inner lid) would be deformed by autoclaving.
Q. What other options are available?
A. We are considering an option to keep the medium cool. For more requests, please feel free to contact us.
Q. How much is the system?
A. The main body (CEME-0200) is $1,844. The disposable component set with two 125ml bottles is $60. With six 15ml tubes, $91.
Q. Are disposable components and bottles sterilized?
A. Yes, both are gamma ray sterilized before shipment.
Swipe left to see the remaining contents
W194 × D228 × H140 mm
|Other Wetted Materials
Silicone, SUS304, or PP
Approximately 500 g (*Medium and AC adaptor weight are not included)
External power supply (AC adaptor) / AA Alkaline Battery
|Applicable Culture Vessel
6 Well Plate*1
|Initially Required Medium Volume (Per Well)
|Volume Replaced (Per Well)
|Medium Replacement Interval
12h / 24h / 48h / 72h
The volume of medium botttle are 125 ml or 15 ml centrifuge Tube.
Note: Details including specifications, etc. may be changed without notice.
*1: Recommended Well Plate: Thermo Fisher Scientific Inc.:NUNC #140675, Sumitomo Bakelite Co., Ltd. MS-80060
*2: Volume may slightly vary depending on the type of well plate. Can be customized.