- Automates culture medium replacement at low cost!
- Suitable for applications where constant and frequent medium replacement is required. (e.g. iPS cell culture)
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Automatic medium replacement
The culture medium is replaced automatically at a specified interval (3, 6, 12, or 24 hours).
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Can be used in an incubator
The system can operate continuously for up to 7 days with batteries, and can be placed in an incubator without any wiring.
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Accommodates a standard 6-well plate
A standard 6-well plate can be used, which reduces running costs and minimizes environmental changes resulting from introducing the system.
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Observable on a microscope
The 6-well plate can remain in the system and be placed on a microscope stage when you observe the cells, which also reduces environmental changes.
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Disposable
All system components that are exposed to the medium are replaceable.
APPLICATION EXAMPLES
Case 1. hiPSC (Human induced Pluripotent Stem Cells)
Colony growth was successfully observed for 4 days after passage (engraftment), during which the culture medium was automatically replaced by our system.
[Experiment Condition]
･Cells: 253G1 Human iPS cells (NHDF reprogrammed by 3 factors; Oct3/4, Sox2, Klf4)
･Feeder Cells: MMC treated SNL76/7 cells
･Medium: DMEM-12 (including KSR, NEAA, PSG, 2-ME, bFGF)
[Data courtesy of Kidoaki S. Lab, Institute for Materials Chemistry and Engineering, Kyushu University (Japan)]
Case 2. Embryoid Bodies (EB) of mouse iPS cells
iPS cells were cultured in Knockout D-MEM based myocardial differentiation medium using our system, and pulsation of differentiation-inducted cardiomyocytes derived from mouse iPS cells was observed.
[Data courtesy of Fujii T. Lab, Institute of Industrial Science, The University of Tokyo (Japan)]
VIDEO OF PORTABLE MEDIUM REPLACEMENT SYSTEM
EXAMPLES OF CUSTOMIZATION
Customization of Medium Bottle
It is recommendable to whom would like to examine culture conditions by putting a different medium in each well. The figure shows an example for a 6-well plate by using six of 15 ml centrifuge tubes. Depending on your request, we can customize any condition such as number of medium and/or shape and volume of the bottles. Please feel free to contact us for details.
Customization of External Power Supply
Do you worry about running out the battery during the operation?
Are you lazy to exchange batteries frequently?
If yes, it is advisable to use the external power supply, instead. With an AC adapter, you can conduct long lasting cell culture without any worry. Please feel free to contact us for details.
FAQ
Q. How much medium can be replaced?
A. The volume of medium is 3.0 ml during culture, of which 2.7 ml or more must be
replaced (per well, using Takasago recommended well plate).
Note: If Takasago recommended well plates are not used, the well depth could be different, resulting in
different replacement medium volume. The medium replacement volume can be customized.
Q. What are the wetted parts made of?
A. SUS304 (nozzle), PP (bottle), PP (straw), silicone (tube).
Q. How frequently is the medium replaced?
A. Every 3, 6, 12 and 24 hours are our basic setting. The interval setting can be customized.
Q. What should be used as the power supply?
A. Two AA alkaline batteries.
Note: Do not use nickel-metal hydride (NiMH) batteries because the controller may be damaged by the gas generated.
Q. What is the capacity of the reservoir bottle?
A. 125 ml, which corresponds to the volume of 5 times replacement.
Q. When should I replace the batteries?
A. We have confirmed 7 consecutive days operation, under 24-hour medium
replacement interval. However, we recommend early replacement.
Q. Which type of well plate is adaptable?
A. Thermo Fisher Scientific Inc.: NUNC # 140675 and Sumitomo Bakelite II:
MS-80060 are our recommendation.
Note: A common 6-well plate can also be used, but the residual medium volume may change.
Q Is there any application example?
A Cardio myogenic differentiation of iPS cells, culture of chicken
cardiomyocytes, and colony formation in undifferentiated iPS cells.
Q. Is it possible to use in a CO2 incubator?
A. Yes.
Q. What is the reliability of replacement fluid volume?
A. ±10% (Per well).
Q. How much is the flow rate of the pump?
A. Approximately 0.8 mL/min per tubing.
Q. Is it possible to autoclave the disposable components and bottles?
A. Basically we do not recommend. Although, autoclavable materials are
basically used the well plate top cover (inner lid) would be deformed by autoclaving.
Q. What other options are available?
A. We are considering an option to keep the medium cool. For more requests,
feel free to contact us.
Q. How much is the system?
A. The main body (CEME-0102) is 150,000 JPY. The disposable component set
is 3,810 JPY. The two bottles set is 1,400 JPY. (Prices excluding tax.)
Q. Are disposable components and bottles sterilized?
A. Yes, both are gamma ray sterilized before shipment.
SPECIFICATIONS
Swipe left to see the remaining contents
| Model Number |
CEME-0102
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| Outer Dimensions |
W194 × D228 × H140 mm
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| Other wetted materials |
Silicone, SUS304, or PP
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| Weight |
Approximately 500 g (*Medium weight is not included)
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| Features |
| Power Supply |
AA Alkaline Battery*2 |
| Applicable Culture Vessel |
6 well plate*1 |
| Initially Required Medium Volume (Per well) |
3.0 mL*2 |
| Volume Replaced(Per well) |
2.7 mL*2 |
| Medium Replacement Interval |
3h/6h/12h/24h |
*1 Recommended well plate: Thermo Fisher Scientific Inc.:NUNC #140675, Sumitomo Bakelite Co., Ltd. MS-80060
*2 Volume may slightly vary depending on the type of well plate.
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